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• A facile, controllable and low-cytotoxic

method of physically depositing and

stabilizing silk fifibroin using HFIP solvent

onto Ti biometal TC4 was established.

• The interface adhesion strength was de

termined through advanced centrifugal

measurement, and high silk fifibroin

(SF)-TC4 adhesion strength of 7 MPa

was achieved with optimized substrate

roughness (Ra~1 μm).

• The water wettability of TC4 was im

proved signifificantly with SF coatings.

• The SF-TC4 coating-substrate system

proved to improve osteoblast cell viabil

ity and growth.